The cylinder is made of polypropylene (PP) and the piston is made of polyethylene (PE). The pistons of the 0.1 ml and 0.2 ml Combitips are made of LCP (Liquid Crystal Polymer).

We are happy to send you a quality certificate for Combitip standard goods. Since Combitips are also available in Eppendorf Biopur quality, a batch-specific Eppendorf Biopur quality certificate is also available. Eppendorf Biopur goods are guaranteed to be sterile and free of pyrogen, RNase, DNA and ATP.

Yes they are. This information is also given on the corresponding certificate.

For this Combitip plus the gray Adapter is needed. Order-Nr. 00630 069.161; Biopur Quality, Order-Nr. 0030 069.480.

The diameters of Combitips which can be used are: 0.1 ml ~ 1.5 mm, 0.2.ml ~ 2.2.mm, 0.5 ml ~ 3.6 mm, 1 ml ~ 5 mm, 2.5 ml ~ 8 mm, 5 ml ~ 11 mm, 10 ml ~ 16 mm, 25 ml ~ 25 mm, 50 ml ~ 36 mm.

No, you can also work with Combitips that are not completely filled. Please note that the 1st dispensing must also be discarded in this case.

These liquids have not been tested separately. The test result for petrol, which contains both these components, shows that use at room temperature is possible for 10 min to several hours. It should therefore be possible to pipette these substances.

Combitips plus are resistant to most moderate inorganic acids. For example, 10% nitric acid, 60% sulphuric acid, 40% hydrofluoric acid and 35% hydrochloric acid can be taken up without damaging the Combitip and can be dispensed over a period of several weeks. We are not, however, able to give any information about dispensing accuracy or functionality of liquid dispensing. Also, we are not able to make any generally applicable statements for organic acids since the resistance varies considerably, depending on the acid. For example, the Combitip is resistant to 50% acetic acid at room temperature, but not to butyric acid.

This substance has not been tested separately. The test result for trichloroacetic acid on PE and PP shows that it is possible to use Combitips plus. Pipetting trifluoroacetic acid ought therefore to be possible too.

The primary components of petroleum spirit are pentane, hexane and octane. Because the secondary components are unknown, we cannot guarantee that the Combitips are suitable for your application. However, we would be pleased to send you a sample of the Combitips to try out.

The Combitips adapter are wear parts. They should be changed regularly. Worn-out adapters might get caught when ejecting the combitip.

Yes. With the "Pierce-it-Lite" heat sealing foil from Eppendorf this is possible without problems!

Yes. We certify our PC plates and sealing options with "PCR-Clean". This certificate stands for guaranteed freedom from human DNA, DNase, RNase and PCR inhibitors.

No, the blister packaging is free from latex.

The composition of the epTIPS Biopur fulfills the requirements of the FDA regulation 21 CFR § 177,1520 "Olefin polymers". Please note: The suitability of a consumer item for the intended purpose, the influence of the filler on taste and smell, and the compliance with limitations (e.g. global migration, specific limitations and other analytical specifications) should be tested by the manufacturer or user for individual cases.

All Biopur products are manufactured fully automatically under cleanroom conditions. This eliminates contamination of the disposables through contact with people. Beta radiation is used as the sterilization method. An independent laboratory checks that each individual batch is free of pyrogens.

Contamination and cross-over can only be avoided by the use of single-use plastic items. In addition there is no annoying and time-consuming cleaning of glassware.

Yes, a general quality certificate is available for epTIPS, which is included in the delivery package.

Like all our single-use plastic items, our pipette tips are also made of polypropylene and can therefore be stored at -10°C even for many years. Please note that you should keep the pipette tips for several minutes at room temperature before pipetting.

Organic, heavy-metal-free dyes (PP granulate) are used to tint the pipette tips.

Yes. Use on a sterile bench, for example, is therefore completely safe.

The tips are electrically neutral. There are no charged particles on the surface. It is therefore unlikely that charged particles like ions or ionic compounds will get caught on the walls.

No.

Yes, this is clear from the details about substances contained in the safety/technical data sheets.

The epTIPS of PP are suitable for liquids with a temperature of max. 90°C.

No, we advise against this application.

The box can be autoclaved up to 100 times, the reloads up to 5 times.

The geometry of the epBox housing changes when the box(es) is (are) forced into the autoclave and the door of the autoclave can only be closed with a degree of force. Once the autoclave has cooled down, the deformed shape is retained. Stacking of many boxes should also be avoided. What is more, autoclaving temperature should be checked regularly. Experience suggests that autoclaves are frequently operated at excessive temperatures. The permissible temperature quoted by Eppendorf should never be exceeded.

No, the epTIPS Box cannot be autoclaved at 133 °C. We recommend 121 °C.

The UV stability of the existing epTIPS Box of polycarbonate and of the existing epTIPS Racks of polypropylene were tested during development. These were thereby irradiated with a lamp of 254 nm and energy of approx. 1 J/cm2 for 16.7 hours. After 10 cycles, thus a total of 167 hours, there were still no signs of adverse effects on the box material. Because the energy of the lamp used is considerably higher than that of a lamp used in a sterile bench, it can be assumed that both boxes can be exposed to UV radiation for at least 300-600 hours in a sterile bench without problems. Because it is well known that polypropylene is at least 3 times more stable against UV radiation than polycarbonate, correspondingly longer UV stability under sterile bench conditions can be presumed for the epTIPS Racks.

These tips will grant an advantage especially during pipetting of solutions containing detergents, such as PCR master mixes, enzyme solutions and many buffers. The LoRetention tips are characterized by significantly improved flow performance, thus achieving more economic use of expensive reagents, while valuable sample material is used effectively. Furthermore, reproducibility is improved, since wetting of the tip is dependent on both user and handling (for example: pipetting speed) and can therefore vary considerably.

The LoRetention tips are made from polypropylene, as are standard tips, and they are resistant to a number of organic solvents. Further information regarding chemical resistance properties of pipette tips can be found in the User Guide no. 23: Chemical Stability of Consumables.

Yes. Foam formation is rarely observed with the use of LoRetention tips. This facilitates the handling of these solutions and increases reproducibility.

No. No silicone is used in the production of Mastertips. The plastics used likewise contain no silicone.

Maxitips are made of polypropylene.

Because the maximum volume of the GELoader Tips amounts to 20 µl, pipettes with a grey button can be used, e.g. Reference 2-20 µl.

The maximum volume of the GELoader Tips is 20 µl.

Yes - Eppendorf Deepwell Plate are optional available with Barcode. Detailed ordering information can be found under www.eppendorf.com/DWP .

No - the plates are neither coated nor siliconized.

Please see for that Plate Dimensions included in the Technical Information (www.eppendorf.com/DWP).

The plates are packed stacked in a resealable ZipLock bag.

At least 5 years at a storage temperature of +5 to +35 °C.

All plates listed in the Eppendorf catalog can be sealed with the heat sealer.

The heat sealer from Eppendorf in combination with heat sealing foils or films offers the best sealing. Another effective method is to use Eppendorf cap strips. For sealing with cap strips, we recommend using the capping aid, an inexpensive tool for producing a very effective seal as cannot be obtained by setting on the caps with the fingers alone.

The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: support@eppendorf.com.

Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.

Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.

Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.

The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.

Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.

Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page www.eppendorf.com/microplates.

"Our tube assortment does not include coated or siliconized tubes. In order to keep sample loss caused by the adsorption of sample material on the tube wall to a minimum, we recommend using the Eppendorf Protein LoBind Tubes or Eppendorf DNA LoBind Tubes. These are made of a special type of polypropylene and are manufactured under optimized process conditions. "

Yes. Please refer to the operating manual.

"No. Bacterial endotoxins (lipopolysaccharide, LPS) are very stable and withstand normal autoclaving at 121 °C. To inactivate LPS, the objects have to be baked at 250 °C, which, of course, is not possible with plastics."

"All micro test tubes designated with "PCR clean" are free of human DNA, DNase, RNase and PCR inhibitors. Our Safe-Lock micro test tubes are also available in the "Eppendorf Biopur" purity standard. Due to the special manufacturing processes and the individual blister-packaging, Biopur products are certified "DNA free"."

"Definition of RT (room temperature): +5 to +35 °C."

"Yes. The tube material could shrink somewhat, but this should have no negative effect on the tube seal. "

"Our 1.5 ml Safe-Lock micro test tubes are suitable for use in the food industry according to the Food and Consumer Goods Legislation § 5, paragraph 1 no. 1 (food and consumer goods). Please note: The suitability of a consumer item for the intended purpose, the influence of the taste and smell of the filled goods and the conformation to limits (e.g. global migration, specific limits and further analytical requirements) must be checked with the manufacturer or user in individual cases."

In the case of a well sealed Eppendorf Safe-Lock micro test tube, evaporation doesn't play a role either at room temperature or at the named temperatures. The material itself has a low level of permeability for steam, but this is insignificant.

"The composition of the 1.5 ml Safe-Lock micro test tubes conforms to the specifications of the FDA regulation 21 CFR § 177,1520 "Olefin polymers". Please note: The suitability of a consumer item for the intended purpose, the influence of the taste and smell of the filled goods and the conformation to limits (e.g. global migration, specific limits and further analytical requirements) must be checked with the manufacturer or user in individual cases."

They can be used from -80 °C to +100 °C. However at temperatures of > 95 °C the tube lids are more likely to pop open. For purposes of autoclaving, the tubes can be heated up to 121 °C.

"Eppendorf Tubes can be stored up to –200°C in the gaseous phase directly above the liquid nitrogen although we can't guarantee that the tubes stay sealed as it is possible that the lid could open accidentally. WARNING! Do not use Eppendorf tubes for storage in the liquid phase of liquid nitrogen. Such use may cause entrapment of liquefied nitrogen inside the vial and lead to pressure build-up resulting in possible explosion or biohazard release. "

In comparison to a Safe-Lock Tube, the DNA LoBind Tube shows significantly less DNA bonding affinity. This effect is most apparent with high salt concentrations and when working with short DNA fragments. The DNA LoBind Tube is therefore especially suitable for applications that should be carried out with the minimum loss of nucleic acid material.

Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimized manucturing process.

"The Micropestle is manufactured from polypropylene."

The Rack 3821 is manufactured from noryl, a polyphenyl ether modified with polystyrene.

No. The propylenes which we use are materials of foodstuff quality. It is tested according to the directives for materials which come into contact with foodstuffs of the German Federal Office of Health and has no nitrogen-containing components.