Mastercycler® gradient:

The Mastercycler gradient is a compact thermal cycler with a 96-position universal block for 96 x 0.2 ml tubes, 77 x 0.5 ml tubes or one 96-well plate. The programmable gradient function enables a temperature gradient of up to 20°C to be generated across the block. During the optimization of a PCR protocol, this allows rapid testing of twelve different temperatures in one single experiment. This, in turn, enables the ideal annealing temperature to be determined rapidly and reliably. State-of-the-art technology, easy-to-use software, and personal cards for storing individually-developed programs ensure unique flexibility for even the most demanding experiments.

A maximum temperature gradient up to 20°C can be programmed across the 96-well sample block. This figure shows an IR thermograph of the Mastercycler block working in the gradient mode. The gradient function of the Mastercycler allows constant ramping to ensure that temperature optimized programs are fully transferable to non-gradient instruments.

Two exclusive features of the Mastercycler gradient are Triple Circuit Technology and Eppendorf’s SteadySlope. Triple Circuit Technology provides an additional heating and cooling zone on the block. This guarantees precise temperature control of all gradient profiles and results in a superior temperature profile compared to other two-zone cyclers. Eppendorf’s SteadySlope ensures that the Mastercycler gradient always works with optimal, constant heating and cooling rates in both the gradient and standard mode. Results from the gradient experiments are thus seamlessly transferred to routine applications.

  • Gradient block: selectable temperature gradient from 1°C to a max. of 20°C
  • Triple Circuit Technology
  • SteadySlope Gradient Technology
  • Heating and cooling via Peltier technology
  • Temperature control range from 4°C to 99°C
  • Heated lid included
  • Eight-line LCD display
  • Utmost flexibility, simple programming: gradient function, adjustable ramps, touch-down, link function, delay, pause function
  • RS-232 and printer interface connections
  • Dimensions: width 26 cm, depth 41 cm, height 27 cm
  • Two-year warranty
Eppendorf Scientific SteadySlope ramp rate remains constant as different parts of the block form the gradient
All-in-one universal block of the Mastercycler gradient for 96 x 0.2 ml tubes, 77 x 0.5 ml tubes or one 96-well plate
The new Mastercycler with its 96-position universal block is the ideal, economical complement to the well-known Mastercycler gradient. Once you have optimized your PCR* using the Mastercycler gradient, you can run your experiments at the same high performance level without losing valuable experimental time on your Mastercycler gradient.As with all other Eppendorf Mastercyclers the new Mastercycler offers fast temperature control, exact block homogeneity and high programming and operation flexibility. Personal cards enable safe program storage as well as easy and reliable transfer of your individual protocols between different Mastercyclers.
  • Universal block for 96 x 0.2 ml tubes, 77 x 0.5 ml tubes or one 8 x 12 microplate
  • Temperature control range from 4 °C to 99 °C
  • Utmost flexibility, simple programming: variable ramps, time and temperature increments,incubation and in situ mode, link and pause function
  • Software supported in situ adapter
  • Heated lid
  • RS 232 interface
  • Autocalibration with temperature validation system
  • Autorestart option in case of power failure
  • Software update via internet
  • 2-year warranty

The Mastercycler personal is a benchtop model designed to meet individual applications. Like the Mastercycler gradient, it offers a high degree of flexibility. Its 25-position universal block can accommodate 25 x 0.2 ml tubes, 16 x 0.5 ml tubes or one microplate in a 5 x 5 grid. The 100-program memory can be extended infinitely with the aid of personal cards, which can each store up to 10 individually developed protocols. The personal card also enables the simple and efficient transfer of methods from one Mastercycler workstation to another, as well as between the Mastercycler personal and gradient. The temperature control range of both instruments extends from 4°C to 99°C. Last but not least, both Mastercyclers are authorized for PCR.